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Reverse transcriptase enzymes / Engineered reverse transcriptases
Engineered reverse transcriptase for cDNA synthesis; glycerol content and concentration vary by formulation. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Reverse transcriptase enzymes / Engineered reverse transcriptases
Engineered reverse transcriptase for cDNA synthesis; glycerol content and concentration vary by formulation. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Reverse transcriptase enzymes / Engineered reverse transcriptases
Engineered reverse transcriptase for cDNA synthesis; glycerol content and concentration vary by formulation. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Library amplification
Master mix for amplification during sequencing-library construction. Check whether platform-specific primers are included. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
DNA ligases / HiFi Taq DNA Ligase
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
DNA ligases / HiFi Taq DNA Ligase
Verify substrate, formulation, activity units, buffers and experimental conditions before substitution.
Streptavidin agarose resins
Streptavidin coupled to agarose for biotin capture. Nonmagnetic support requires an appropriate separation method.
High-fidelity PCR / Kits
High-fidelity PCR core kit includes enzyme, nucleotides and reaction buffers. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
High-fidelity PCR / Standalone enzymes
Proofreading DNA polymerase or polymerase blend for high-fidelity PCR. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
High-fidelity PCR / Standalone enzymes
Standalone proofreading or proofreading-blend DNA polymerase for high-fidelity endpoint PCR. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Microbial DNA extraction / Spin-column purification
Silica-membrane purification isolates genomic DNA from Gram-positive and Gram-negative bacteria. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.
Genomic DNA extraction / Spin-column purification
Silica-based extraction purifies genomic DNA from blood, body-fluid fractions and cultured cells. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.
Plant DNA extraction / Spin-column purification
Silica-membrane extraction of plant genomic DNA with removal of polysaccharides and polyphenols. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.
Genomic DNA extraction / Spin-column purification
Silica mini spin columns isolate genomic DNA from human and animal saliva. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.
Fecal and soil DNA extraction / Spin-column purification
Bead disruption and silica-membrane purification isolate DNA from soil while removing humic acids and PCR inhibitors. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.
Fecal and soil DNA extraction / Spin-column purification
Silica-membrane purification of stool DNA includes inhibitor-removal chemistry. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.
Genomic DNA extraction / Spin-column purification
Spin columns isolate genomic DNA from fresh or preserved swab samples. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.
Genomic DNA extraction / Spin-column purification
Silica-membrane purification of genomic DNA from animal tissue, blood, bacteria, yeast and fungi. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.
Tissue and insect RNA extraction / Spin-column purification
Mini-column purification of total RNA from animal tissue; manufacturer reports yield up to 100 µg. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.
Yeast RNA extraction / Spin-column purification
Silica spin-column purification of total RNA from tissues, cultured cells, bacteria, yeast and fungi. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.
Yeast DNA extraction / Spin-column purification
Lyticase and bead disruption followed by silica columns purify genomic DNA from yeast and fungal samples. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.
Reverse transcriptase enzymes / Engineered reverse transcriptases
Engineered M-MLV-derived reverse transcriptase with increased thermal stability; formulation and inhibitor tolerance require protocol review. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
HincII restriction enzymes / Standard formulations
Same enzyme identity candidate. Verify recognition and cleavage specificity, methylation sensitivity, reaction buffer, concentration and heat inactivation in the source before substitution.
HincII restriction enzymes / Standard formulations
Same enzyme identity candidate. Verify recognition and cleavage specificity, methylation sensitivity, reaction buffer, concentration and heat inactivation in the source before substitution.
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