Cells & expression

Cloning E. coli / 5-alpha strains

Chemically competent 5-alpha cloning cells. Verify full genotype and transformation efficiency.

Counterselection reagents / 5-Fluoroorotic acid

Candidate category only. Match the stated sample, material, capacity, geometry and required output before comparison; experimental interchangeability is not established.

Counterselection reagents / 5-Fluoroorotic acid

Account for hydrate form, purity and molecular weight.

Expression inducers / L-arabinose

Candidate category only. Match the stated sample, material, capacity, geometry and required output before comparison; experimental interchangeability is not established.

Mating pheromones / a-factor

Candidate category only. Match the stated sample, material, capacity, geometry and required output before comparison; experimental interchangeability is not established.

Mating pheromones / Alpha-factor

Candidate category only. Match the stated sample, material, capacity, geometry and required output before comparison; experimental interchangeability is not established.

Mating pheromones / Alpha-factor

TFA salt; distinguish alpha-factor from lipid-modified a-factor.

Antibiotic selection / Ampicillin solutions

Sterile ampicillin sodium stock solution at 100 mg/ml for plasmid selection. Match working concentration.

Antibiotic selection / Ampicillin solutions

Ampicillin antibiotic selection solution. Confirm concentration, solvent and working dilution.

Antibiotic selection / Ampicillin solutions

Candidate category only. Match the stated sample, material, capacity, geometry and required output before comparison; experimental interchangeability is not established.

Antibiotic selection / Ampicillin solutions

Adjust for stock concentration and solvent requirements.

Antibiotic selection / Ampicillin solutions

Adjust for stock concentration and solvent requirements.

E. coli chemical lysis reagents

Mild nonionic-detergent bacterial lysis reagent in Tris buffer. Does not itself contain enzymes; validate extraction efficiency and add lysozyme or nuclease when needed.

Protein expression E. coli / BL21 strains / BL21(DE3) chemically competent cells

Chemically competent BL21(DE3) cells with T7 expression capability; lon and ompT deficient, phage T1 resistant. Compare host genotype and induction conditions.

Protein expression E. coli / BL21 strains / BL21 chemically competent cells without DE3

Chemically competent BL21 cells without DE3 for non-T7 expression vectors. lon and ompT deficient; this is not a T7-polymerase expression host.

Protein expression E. coli / BL21 strains / BL21 chemically competent cells without DE3

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Protein-expression E. coli BL21; DE3/T7 requirements must be matched.

Protein expression E. coli / BL21 strains / BL21 chemically competent cells without DE3

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Protein expression E. coli / BL21 strains / BL21 chemically competent cells without DE3

Chemically competent BL21 cells without T7 RNA polymerase expression. Intended for compatible non-T7 expression systems.

Protein expression E. coli / T7 expression strains / BL21(DE3) chemically competent cells

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. DE3 lysogen supplies T7 RNA polymerase; NEB T7 Express variants have different genotype and toxicity controls.

Protein expression E. coli / BL21 strains / BL21(DE3) chemically competent cells

BL21(DE3) chemical transformation cells for T7-promoter expression. No pLysS plasmid is specified; follow the supplier direct-plating protocol.

Protein expression E. coli / BL21 strains / BL21(DE3) chemically competent cells

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Antibiotic selection / Chloramphenicol solutions

Different stock concentration and solvent may affect experimental use.

Antibiotic selection / Chloramphenicol solutions

Chloramphenicol stock solution at 50 mg/ml. Adjust working dilution and verify solvent compatibility.

Antibiotic selection / Chloramphenicol solutions

Candidate category only. Match the stated sample, material, capacity, geometry and required output before comparison; experimental interchangeability is not established.