Competent cells

Cloning E. coli / 5-alpha strains

Chemically competent 5-alpha cloning cells. Verify full genotype and transformation efficiency.

Protein expression E. coli / BL21 strains / BL21(DE3) chemically competent cells

Chemically competent BL21(DE3) cells with T7 expression capability; lon and ompT deficient, phage T1 resistant. Compare host genotype and induction conditions.

Protein expression E. coli / BL21 strains / BL21 chemically competent cells without DE3

Chemically competent BL21 cells without DE3 for non-T7 expression vectors. lon and ompT deficient; this is not a T7-polymerase expression host.

Protein expression E. coli / BL21 strains / BL21 chemically competent cells without DE3

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Protein-expression E. coli BL21; DE3/T7 requirements must be matched.

Protein expression E. coli / BL21 strains / BL21 chemically competent cells without DE3

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Protein expression E. coli / BL21 strains / BL21 chemically competent cells without DE3

Chemically competent BL21 cells without T7 RNA polymerase expression. Intended for compatible non-T7 expression systems.

Protein expression E. coli / T7 expression strains / BL21(DE3) chemically competent cells

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. DE3 lysogen supplies T7 RNA polymerase; NEB T7 Express variants have different genotype and toxicity controls.

Protein expression E. coli / BL21 strains / BL21(DE3) chemically competent cells

BL21(DE3) chemical transformation cells for T7-promoter expression. No pLysS plasmid is specified; follow the supplier direct-plating protocol.

Protein expression E. coli / BL21 strains / BL21(DE3) chemically competent cells

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Cloning E. coli / Methylation-deficient strains

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Chemically competent E. coli / 10B

Chemically competent DH10B cells with mcrA and mrr-hsdRMS-mcrBC deletions, suitable for methylated-DNA cloning. Verify genotype and DNA input requirements.

Cloning E. coli / 10-beta strains

Chemically competent DH10beta cloning cells. Compare genotype, efficiency and DNA size tolerance.

Chemically competent E. coli / DH5 Alpha

Chemically competent DH5-alpha cells with recA1 and endA1 for routine cloning and plasmid propagation. Check genotype and transformation protocol.

Chemically competent E. coli / DH5 Alpha

Verify full genotype, efficiency and transformation requirements.

Chemically competent E. coli / 10B

Chemically competent DH10B cells using the DirectPlate transformation workflow. Verify genotype and plasmid compatibility. Manufacturer recommends its specific mix-and-plate protocol rather than conventional heat shock.

Protein expression E. coli / BL21 strains / BL21(DE3) chemically competent cells

Chemically competent BL21(DE3) cells using the DirectPlate transformation workflow. Verify genotype and plasmid compatibility. Manufacturer recommends its specific mix-and-plate protocol rather than conventional heat shock.

Chemically competent E. coli / DH5 Alpha

Chemically competent DH5-alpha cells using the DirectPlate transformation workflow. Verify genotype and plasmid compatibility. Manufacturer recommends its specific mix-and-plate protocol rather than conventional heat shock.

Chemically competent E. coli / TG1

Chemically competent TG1 cells using the DirectPlate transformation workflow. Verify genotype and plasmid compatibility. Manufacturer recommends its specific mix-and-plate protocol rather than conventional heat shock.

Chemically competent E. coli / 10B

Chemically competent DH10B cells using the DirectPlate transformation workflow. Verify genotype and plasmid compatibility. Manufacturer recommends its specific mix-and-plate protocol rather than conventional heat shock.

Cloning E. coli / 5-alpha strains

Chemically competent 5-alpha cloning cells. Match genotype and transformation efficiency for the intended plasmid.

Electrocompetent E. coli / 10-beta strains

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Electrocompetent DH10B-type cloning cells; use electroporation, not heat shock.

Cloning E. coli / 10-beta strains

Chemically competent GB10B cells described by the manufacturer as DH10B-equivalent. Compare full genotype, DNA size and transformation efficiency.

Electrocompetent E. coli / 10-beta strains

Electrocompetent DH10B-equivalent cloning cells. Confirm electrical transformation protocol and efficiency for the intended construct.

Electrocompetent E. coli / 10-beta strains

High-efficiency electrocompetent DH10B-equivalent cells for demanding and large-construct cloning applications. Verify insert-size requirements.