I-SceI restriction enzymes / Standard formulations
Same enzyme identity candidate. Verify recognition and cleavage specificity, methylation sensitivity, reaction buffer, concentration and heat inactivation in the source before substitution.
Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Cold-active TEV protease; compare activity at chosen incubation temperature.
Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. IgG-specific IdeZ protease; assess species and subclass cleavage.
Zymo 10B genotype equivalence is not established; compare complete strain genotype and transformation chemistry. Full genotype equivalence to Zymo 10B is unverified.
Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. O-glycan-dependent protease. Cleavage-site profile must match application.
Liquid SYBR Green qPCR formulation; reference dye, hot-start chemistry and optional carryover prevention differ by product and require protocol review. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.