Cells & expression

Chemically competent E. coli / HB101

Compare full genotype and transformation efficiency.

Chemically competent E. coli / HB101

HB101 chemical transformation cells with recA13 and hsdS20 genotype. Does not support alpha-complementation; follow the supplier no-heat-shock workflow.

Chemically competent E. coli / 10B

Zymo 10B genotype equivalence is not established; compare complete strain genotype and transformation chemistry. Full genotype equivalence to Zymo 10B is unverified.

Expression inducers / IPTG

Match purity and formulation.

Expression inducers / IPTG

IPTG expression inducer supplied as a solid. Prepare the working solution and induction concentration required by the host and promoter.

Expression inducers / IPTG

Candidate category only. Match the stated sample, material, capacity, geometry and required output before comparison; experimental interchangeability is not established.

Chemically competent E. coli / JM109

Compare full genotype and transformation efficiency; handling is vendor-specific.

Cloning E. coli / Methylation-deficient strains

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Dam-negative and Dcm-negative host for unmethylated plasmid preparation; other genotype differences remain.

Yeast cells / K. lactis competent cells

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

K. lactis expression kits

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Antibiotic selection / Kanamycin solutions

Kanamycin monosulfate stock solution at 50 mg/ml. Match working selection concentration and resistance marker.

Antibiotic selection / Kanamycin solutions

Kanamycin stock solution at 100 mg/ml for selection. Adjust working dilution and verify sterilization and formulation.

Antibiotic selection / Kanamycin solutions

Match stock concentration.

Antibiotic selection / Kanamycin solutions

Match stock concentration.

Antibiotic selection / Kanamycin solutions

Candidate category only. Match the stated sample, material, capacity, geometry and required output before comparison; experimental interchangeability is not established.

Expression inducers / L-arabinose

Match purity and final concentration.

Expression inducers / L-arabinose

L-arabinose inducer of the AraC-regulated PBAD promoter. Match concentration and medium carbon-source conditions.

Protein expression E. coli / Lemo21 strains

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Helper phages / M13KO7 helper phage

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Helper phages / M13KO7 helper phage

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Helper phage for phagemid rescue; does not replace phage display peptide libraries.

Cloning E. coli / 10-beta strains

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. DH10B-type chemical transformation for large plasmids. NEB 10-beta and DH10B genotypes are not asserted identical.

Chemically competent E. coli / HB101

Candidate category only. Match the stated sample, material, capacity, geometry and required output before comparison; experimental interchangeability is not established.

Chemically competent E. coli / JM109

Candidate category only. Match the stated sample, material, capacity, geometry and required output before comparison; experimental interchangeability is not established.

Chemically competent E. coli / TG1

Candidate category only. Match the stated sample, material, capacity, geometry and required output before comparison; experimental interchangeability is not established.