T4 DNA Ligase
Read or write a reviewT4 DNA ligases / Liquid enzymes
T4 DNA ligase for blunt- and cohesive-ended DNA ligation. Confirm formulation, activity units and experimental conditions before substitution.
BIOLOGICAL DOMAIN
DNA engineering reagents support sequence-specific cutting, end modification, ligation, assembly and genome editing workflows.
Start with the change you want to make to DNA. Restriction digestion, ligation, assembly and end modification perform different steps, while genome editing introduces additional targeting requirements. Narrower categories distinguish enzyme identity, reaction role and formulation. A shared enzyme name alone does not establish matching cleavage behavior, buffer requirements or activity units.
Choose the required molecular activity before comparing substrate specificity, reaction conditions and supplied components.
Kinases, Phosphatases, Blunt-end preparation
48 productsDNA ligases, T4 DNA ligases, RNA ligases
77 productsProtelomerases, Helicases, Reverse gyrases
10 productsUracil excision, DNA repair enzymes, Base excision repair
32 productsCas9 reagents, Cas12 nucleases, Mutation detection
22 productsSite-specific recombinases, Recombination proteins
8 productsDNA polymerases, DNA polymerase fragments, Terminal transferases
27 productsDNA-degrading nucleases, Structure-specific nucleases, Exonucleases
113 productsSingle-stranded DNA binding
4 productsPCR cloning, Overlap assembly, Type IIS assembly
13 productsSite-directed mutagenesis
4 productsAatII restriction enzymes, Acc65I restriction enzymes, AccI restriction enzymes
942 productsDNA glucosylation
1 productsT4 DNA ligases / Liquid enzymes
T4 DNA ligase for blunt- and cohesive-ended DNA ligation. Confirm formulation, activity units and experimental conditions before substitution.
T4 DNA ligases / Liquid enzymes
Verify substrate, formulation, activity units, buffers and experimental conditions before substitution.
T4 DNA ligases / Liquid enzymes
Official manufacturer product page identifies the enzyme, formulation and catalog code. Supplier reaction buffers and activity definitions must be checked. Confirm formulation, activity units and experimental conditions before substitution.

T4 DNA ligases / Immobilized enzymes
Recombinant T4 DNA ligase is covalently immobilized on 1.0 micrometer magnetic beads. This is an immobilized ligase candidate, not a soluble-enzyme substitute. Confirm formulation, activity units and experimental conditions before substitution.

T4 DNA ligases / Liquid enzymes
High-concentration T4 DNA ligase with a PEG-containing rapid ligation buffer; check enzyme-unit definitions before substitution. Review the manufacturer protocol before substituting.

T4 DNA ligases / Liquid enzymes
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

DNA polymerases / T4 DNA polymerase
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

DNA polymerases / T4 DNA polymerase
Verify substrate, formulation, activity units, buffers and experimental conditions before substitution.
DNA polymerases / T4 DNA polymerase
Verify substrate, formulation, activity units, buffers and experimental conditions before substitution.
DNA polymerases / T4 DNA polymerase
Official manufacturer catalog lists this exact named enzyme and catalog code with a product-page hyperlink. Product-page body was unavailable due to a locale redirect; verify detailed conditions with the supplier. Confirm the supplier protocol, formulation and activity units before substitution.

DNA polymerases / T4 DNA polymerase
T4 DNA polymerase has polymerase and proofreading exonuclease activities for DNA synthesis and end processing. Confirm formulation, activity units and experimental conditions before substitution.
DNA polymerases / T4 DNA polymerase
T4 DNA polymerase with polymerase and proofreading activities for end repair and DNA synthesis. Confirm formulation, activity units and experimental conditions before substitution.

Structure-specific nucleases / T4 PDG (T4 Endonuclease V)
Verify substrate, formulation, activity units, buffers and experimental conditions before substitution.
Structure-specific nucleases / T4 PDG (T4 Endonuclease V)
Official manufacturer catalog lists this exact named enzyme and catalog code with a product-page hyperlink. Product-page body was unavailable due to a locale redirect; verify detailed conditions with the supplier. Confirm the supplier protocol, formulation and activity units before substitution.

Single-stranded DNA binding / T4 gene 32 protein
Verify substrate, formulation, activity units, buffers and experimental conditions before substitution.

Single-stranded DNA binding / T4 gene 32 protein
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Structure-specific nucleases / T4 PDG (T4 Endonuclease V)
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

DNA glucosylation / T4 beta-glucosyltransferase
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
Kinases / T4 Polynucleotide Kinase
Same application category. Compare formulation, input requirements and protocol before substitution.

Kinases / Polynucleotide Kinase
Verify substrate, formulation, activity units, buffers and experimental conditions before substitution.
Kinases / T4 Polynucleotide Kinase
Official manufacturer catalog lists this exact named enzyme and catalog code with a product-page hyperlink. Product-page body was unavailable due to a locale redirect; verify detailed conditions with the supplier. Confirm the supplier protocol, formulation and activity units before substitution.

Kinases / T4 Polynucleotide Kinase
T4 PNK has kinase and 3-prime-phosphatase activities; this is not the phosphatase-minus variant. Confirm formulation, activity units and experimental conditions before substitution.
Phosphatases / T4 Polynucleotide Kinase (PNK) (3' phosphatase minus)
Verify substrate, formulation, activity units, buffers and experimental conditions before substitution.

Phosphatases / T4 Polynucleotide Kinase (PNK) (3' phosphatase minus)
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
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ALTERNATIVE DOES NOT MEAN IDENTICAL
Final groups are designed around experimental substitution requirements. Group membership is provisional until the necessary technical details have been verified for the intended experiment.
Start with your application, then verify the technical requirements against each manufacturer’s documentation.
Broader catalog resources for this branch. These links are discovery starting points, not verified replacements for individual products.