RNA purification

Blood RNA extraction / Spin-column purification

Maxi format: compare input volume and capacity.

Fecal and soil RNA extraction / Spin-column purification

Compare input quantity, recovery range, downstream compatibility and required accessories before substitution.

Phenol and guanidinium extraction reagents

Validate compatibility with the intended cleanup kit and sample.

FFPE RNA extraction / Spin-column purification / FFPE RNA extraction kits

Compare fixation conditions and fragment-size recovery.

Plant RNA extraction / Spin-column purification / Plant total RNA kits

Silica spin-column total RNA extraction from a variety of plant tissues; no small-RNA retention claim. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

RNA extraction / Spin-column purification / RNA longer than 200 nt / Cell and tissue miniprep kits

Silica spin-column RNA extraction from cells, tissue, blood cells, bacteria, yeast and insects; isolates RNA longer than 200 nt. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

Viral nucleic acid extraction / Spin-column purification

Silica spin-column viral DNA and RNA purification; 200 µL sample input. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

Phenol-lysate RNA purification / 96-well silica-plate purification

96-well glass-fiber plate recovery of total RNA from phenol/guanidine GENEzol lysates. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

Microbial RNA extraction / Spin-column purification

Bacterial RNA isolation combines GENEzol phenol/guanidine lysis with spin-column purification and DNase. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

Microbial RNA extraction / Spin-column purification

Bacterial RNA isolation combines GENEzol phenol/guanidine lysis with spin-column purification; without DNase. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

Phenol and guanidinium extraction reagents

Phenol and guanidine isothiocyanate reagent for RNA extraction. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

Phenol-lysate RNA purification / Spin-column purification

GENEzol phenol/guanidine lysis with spin-column total RNA purification and DNase. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

Phenol-lysate RNA purification / Spin-column purification

GENEzol phenol/guanidine lysis with spin-column total RNA purification, without DNase. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

mRNA enrichment / Magnetic bead purification

Oligo-dT-conjugated magnetic beads capture polyadenylated mRNA from total RNA; dT length is not specified, so not assigned to dT25-specific leaves. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

RNA cleanup / Magnetic bead purification

Magnetic-bead RNA cleanup for total RNA, in vitro transcripts, labeled RNA and synthetic RNA. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

RNA extraction / Magnetic bead purification

Magnetic-bead total RNA extraction from animal and plant tissues and cells; supports automated platforms. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

Tissue and insect RNA extraction / Spin-column purification

Mini-column purification of total RNA from animal tissue; manufacturer reports yield up to 100 µg. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.

Yeast RNA extraction / Spin-column purification

Silica spin-column purification of total RNA from tissues, cultured cells, bacteria, yeast and fungi. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.

Yeast RNA extraction / Spin-column purification

Verify lysis for the specific fungal or bacterial organism.

Blood RNA extraction / Spin-column purification

Spin-column leukocyte RNA isolation from blood following selective red-cell lysis. Candidate for the specified application; follow the manufacturer protocol and validate sample and instrument compatibility.

RNA extraction / Magnetic bead purification

Magnetic-bead RNA extraction from PAXgene-stabilized blood with a genomic-DNA filtration step. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.

RNA extraction / Magnetic bead purification

Magnetic-bead extraction of total RNA from cells and tissue in a 96-well workflow. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.

RNA extraction / Magnetic bead purification

Magnetic-bead isolation of total RNA including small RNAs with on-bead DNase treatment. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.

PCR cleanup / Magnetic bead purification

Magnetic-bead cleanup and size selection of DNA and RNA for NGS workflows; bead ratios determine recovered fragment sizes. Candidate for this workflow; follow the supplier protocol and validate input and instrument compatibility.