Glycobiology

Glycan modification enzymes / Endo S glycosidases

IgG-specific Endo S hydrolysis of Fc N-glycans. Match native IgG substrate and glycan sensitivity; no Endo S2 substitution is implied.

Glycan modification enzymes / Galactosidases / Endo-beta-galactosidase

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Glycan modification enzymes / Galactosidases / Endo-beta-galactosidase

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Hydrolyses internal beta-galactose linkages in susceptible glycans.

Glycan modification enzymes / Endo S glycosidases

Endo S for IgG glycan hydrolysis. IgG subclass, species and glycan composition influence digestion and should be checked.

Glycan modification enzymes / Endo D glycosidases

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Endo D glycosidase activity; verify glycan substrate specificity.

N-glycan release / Endo F2

Endo F2 glycosidase for susceptible biantennary complex N-glycans. Verify specificity and conditions for the glycoprotein under study.

N-glycan release / Endo F3

Endo F3 glycosidase for susceptible complex N-glycan structures. Confirm core-fucose dependence and substrate accessibility.

N-glycan release / Endo H

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Endo H activity for native or denatured susceptible glycoproteins.

N-glycan release / Endo H

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Endo H release of susceptible high-mannose/hybrid N-glycans.

Glycan modification enzymes / Deglycosylation enzyme mixes

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Enzyme mixture for protein deglycosylation; check component coverage.

N-glycan release / PNGase F / Rapid formulations

Rapid recombinant PNGase F without glycerol for N-glycan removal prior to chromatography or MS. The rapid buffer protocol must be matched; non-reducing suitability is not assumed.

Glycoprotein substrates

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Glycoprotein substrates

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Glycoprotein substrate and standard; glycoforms vary by preparation.

Glycan modification enzymes / Galactosidases / beta 1-3,4 Galactosidase

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Beta-galactosidase mixture removes beta1-3,4 terminal galactose.

Glycan modification enzymes / Galactosaminidases

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Alpha-N-acetylgalactosaminidase for terminal GalNAc removal.

Glycan modification enzymes / Mannosidases / a 1-2,3,6 Mannosidase

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Broad terminal alpha-mannose linkage specificity.

Glycan modification enzymes / Glycoproteases

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. O-glycan-dependent protease. Cleavage-site profile must match application.

Glycan modification enzymes / Fucosidases / alpha 1-3,4 Fucosidase

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Linkage-specific glycan fucosidase kit.

Glycan modification enzymes / Hexosaminidases

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Glycan modification enzymes / Glucosaminidases

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Removes terminal beta-linked N-acetylglucosamine.

N-glycan release / PNGase A

Native almond glycopeptidase A for N-glycan release from glycopeptides. Compare native substrate accessibility and required proteolytic pretreatment with recombinant PNGase A protocols.

Glycan modification enzymes / Sialidases / alpha 2-3,6,8,9 Neuraminidase A

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Broad alpha2-3,6,8,9 sialic-acid release.

Glycan modification enzymes / Sialidases / alpha 2-3 Neuraminidase S

Candidate only. Verify activity, specificity, formulation and protocol compatibility before substitution. Positionally selective alpha2-3 sialic acid release.

Glycan modification enzymes / Glycoproteases

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.