Q5-XT Hot Start High-Fidelity 2X Master Mix from New England Biolabs is listed in High-fidelity PCR: Master mixes: PCR mixes without specified loading dye.
Pack sizes & catalog numbers
100 rxns (2x1.25ml) · M2499S
PRODUCT OVERVIEW
Endpoint PCR workflows amplify DNA for analysis after thermal cycling.
For this workflow, compare target length, fidelity requirements, hot-start format and supplied reaction components. Use the specifications and official documentation below to assess this product against your protocol.
The indexed manufacturer catalog numbers include M2499S, M2499L.
Compare with context
EXPLORE YOUR OPTIONS
13 cross-manufacturer candidates and 6 same-manufacturer related products.
These products share a candidate substitution group. Interchangeability has not yet been validated against all experimental requirements. Check the listed selection criteria before switching.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
2x Pfu proofreading PCR mix for targets up to approximately 3-4 kb; no separate polymerase dosing required. Review the manufacturer protocol before substituting.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
High-fidelity PCR master mix; loading dye is not specified in the reviewed formulation. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Two-fold concentrated proofreading PCR master mix with buffer, magnesium and dNTPs; no loading dye is specified. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
5x blend of hot-start Taq and a proofreading polymerase; reported fidelity is approximately five times Taq. Review the manufacturer protocol before substituting.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Ready-to-use proofreading PCR formulation with magnesium and dNTPs; no loading dye specified. Hot-start behavior and cycling requirements are formulation-specific. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Ready-to-use proofreading PCR formulation with magnesium and dNTPs; no loading dye specified. Hot-start behavior and cycling requirements are formulation-specific. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Ready-to-use proofreading PCR formulation with magnesium and dNTPs; no loading dye specified. Hot-start behavior and cycling requirements are formulation-specific. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Ready-to-use proofreading PCR formulation with magnesium and dNTPs; no loading dye specified. Hot-start behavior and cycling requirements are formulation-specific. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Catalog category candidate. Experimental interchangeability has not been validated.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
5x chemically activated proofreading PCR mix; manufacturer specifies 12 kb genomic and 24 kb lambda targets. Review the manufacturer protocol before substituting.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
High-fidelity PCR master mix; loading dye is not specified in the reviewed formulation. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
Ready-to-use proofreading PCR formulation with magnesium and dNTPs; no loading dye specified. Hot-start behavior and cycling requirements are formulation-specific. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.
High-fidelity PCR / Master mixes / PCR mixes without specified loading dye
High-fidelity PCR master mix; loading dye is not specified in the reviewed formulation. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Broader catalog resources for this branch. These links are discovery starting points, not verified replacements for individual products.
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Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
Primary classification is an editorial interpretation of the product name and cited source. Technical equivalence is not established by category membership. Read our methodology.