About Standalone enzymes
Endpoint PCR workflows amplify DNA for analysis after thermal cycling.
Standalone enzyme preparations are separated from complete kits and master mixes. Check which buffers, primers, nucleotides and other components must be supplied separately for the protocol.
When selecting products, compare target length, fidelity requirements, hot-start format and supplied reaction components.
Products in this final group are candidates for comparison. Check the individual specifications and manufacturer protocols before substituting one product for another.
Compare with context
Your selection checklist
- 01Template length and GC content
- 02Fidelity, hot-start mechanism and cycling
- 03Detection chemistry and instrument support
- 04Reaction volume and included components
ALTERNATIVE DOES NOT MEAN IDENTICAL
What to check before choosing a product
Final groups are designed around experimental substitution requirements. Group membership is provisional until the necessary technical details have been verified for the intended experiment.
Start with your application, then verify the technical requirements against each manufacturer’s documentation.