Vazyme
HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Add primers. Select a random, oligo(dT), or gene-specific primer for the RT step; the kit also includes gDNA removal.
REAGENT SUBTYPE
RT master mixes
RM-C-08.003.004Compare RT master mixes within Reverse transcription. This category groups catalog records by experimental role and format.
55 products20 manufacturers5 branches
About RT master mixes
Reverse transcription workflows convert RNA into complementary DNA for downstream amplification or analysis.
When selecting products, compare RNA input, primer composition, genomic DNA removal and downstream assay compatibility.
Use the narrower categories to separate experimental requirements before comparing individual products. Products across this broader branch can serve different steps or use different formats.
Explore 5 narrower categories
Mixed-primer RT mixes
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17 productsRT mixes with gDNA removal
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26 productsRT mixes with unspecified priming
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5 productsPrimer-free RT mixes
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6 productsOligo(dT)-primed RT mixes
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1 productsCompare products in this branch
55 productsVazyme
HiScript III RT SuperMix for qPCR (+gDNA wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Pre-primed. A separate 4× gDNA wiper precedes addition of the 5× RT supermix.
Vazyme
HiScript IV All-in-One Ultra RT SuperMix for qPCR
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. Single-reaction gDNA removal and reverse transcription.
Vazyme
HiScript IV RT SuperMix for qPCR (+gDNA wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. Fourth-generation HiScript RT supermix with a gDNA wiper.
Vazyme
HiScript Q RT SuperMix for qPCR (+gDNA wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. Original HiScript Q series with a gDNA wiper workflow.
Vazyme
HisyGo All-in-One RT Red SuperMix for qPCR (+gDNA Wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. All-in-one configuration of the HisyGo RT Red range.
Vazyme
HisyGo RT Red SuperMix for qPCR (+gDNA Wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. Red-format RT mix listed in Vazyme’s qPCR-directed cDNA synthesis catalog.
Bio-Rad
iScript gDNA Clear cDNA Synthesis Kit
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Mixed primers. Contains the iScript RT supermix plus DNase reagents for pretreatment. An alternative kit configuration.
Bio-Rad
iScript Reverse Transcription Supermix for RT-qPCR
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. 100 reactions. A matched no-RT control is supplied for control reactions.
New England Biolabs
LunaScript RT SuperMix
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
New England Biolabs
LunaScript RT SuperMix Kit
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
Takara Bio
PrimeScript RT Master Mix (Perfect Real Time)
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. 5× mix containing random hexamers and oligo(dT). The listed 200 reactions use a 10 µL format.
Quantabio
qScript cDNA SuperMix
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. 5× mix for short targets. The vendor lists 10 pg-1 µg total RNA.
Quantabio
qScript XLT cDNA SuperMix
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. Uses an engineered reverse transcriptase with reduced RNase H activity. Check its own incubation program.
Thermo Scientific
RevertAid Master Mix
Read or write a reviewRT master mixes / RT mixes with unspecified priming
Catalog category candidate. Experimental interchangeability has not been validated.
Thermo Scientific
RevertAid Master Mix with DNase I
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Catalog category candidate. Experimental interchangeability has not been validated.
TOYOBO
ReverTra Ace qPCR RT Master Mix
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. 5× mix supplied with no-RT control. Optimized for short cDNA targets in real-time PCR.
TOYOBO
ReverTra Ace qPCR RT Master Mix with gDNA Remover
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Mixed primers. Sequential DNA removal and RT. TOYOBO specifies its own RT Master Mix II for this workflow.
MedChemExpress
RT Master Mix for qPCR
Read or write a reviewRT master mixes / RT mixes with unspecified priming
See datasheet. Original first-strand formulation for downstream PCR or qPCR.
MedChemExpress
RT Master Mix for qPCR (gDNA digester plus)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Mixed primers. A 2× RT mix follows a separate digestion step. The stock concentration changes the volume added per reaction.
MedChemExpress
RT Master Mix for qPCR II
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. 4× Super RT Mix supplied with water. Compare reagent volume when changing from a 5× mix.
MedChemExpress
RT Master Mix for qPCR II (gDNA digester plus)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. Second-generation RT formulation supplied for workflows requiring removal of residual genomic DNA.
GDSBio
RT-PCR Mix for qPCR
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Reverse-transcription premix includes genomic DNA digestion reagents. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Solis BioDyne
SOLIScript RT cDNA Synthesis Mix (without primers)
Read or write a reviewRT master mixes / Primer-free RT mixes
Primer-free RT reaction premix with a separate SOLIScript/RNase-inhibitor enzyme mix; supply application-specific primers. Review the manufacturer protocol before substituting.
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Your selection checklist
- 01Primer composition and RNA input
- 02Genomic DNA removal and no-RT controls
- 03Reaction volume and incubation program
- 04Downstream PCR or qPCR compatibility
ALTERNATIVE DOES NOT MEAN IDENTICAL
What to check before choosing a product
Final groups are designed around experimental substitution requirements. Group membership is provisional until the necessary technical details have been verified for the intended experiment.
Start with your application, then verify the technical requirements against each manufacturer’s documentation.
Explore manufacturer resources
Broader catalog resources for this branch. These links are discovery starting points, not verified replacements for individual products.