Reverse transcription

RT master mixes / RT mixes with gDNA removal

Reverse-transcription premix includes genomic DNA digestion reagents. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.

RT master mixes / RT mixes with unspecified priming

Pre-primed. Standard first-strand master mix. Select G592 when the integrated DNase formulation is required.

RT master mixes / Mixed-primer RT mixes

Mixed primers. 5× mix for cDNA used in dye- or probe-based qPCR. The manufacturer directs long-amplicon users to another kit.

RT master mixes / RT mixes with gDNA removal

Mixed primers. Compare the gDNA removal workflow with the standard RK20428 mix.

RT master mixes / RT mixes with gDNA removal

Mixed primers. 4× RT mix. A separate remover is added to the same reaction tube.

Template-switching RT

Reverse-transcription enzyme mix supports template switching with a compatible template-switching oligonucleotide. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.

RT master mixes / Oligo(dT)-primed RT mixes

Catalog category candidate. Experimental interchangeability has not been validated.

RT master mixes / Primer-free RT mixes

Catalog category candidate. Experimental interchangeability has not been validated.

RT master mixes / Primer-free RT mixes

Add primers. Use the RNase H-minus format with a separately selected RT primer.

RT master mixes / Primer-free RT mixes

Catalog category candidate. Experimental interchangeability has not been validated.

RT master mixes / RT mixes with gDNA removal

Mixed primers. Premixed temperature-sensitive DNase; the manufacturer specifies a distinct digestion and RT program.

Reverse transcriptase enzymes / AMV reverse transcriptases

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

Reverse transcriptase enzymes / AMV reverse transcriptases

Candidate for the stated function. Confirm substrate, formulation, reaction conditions and application before substitution.

Reverse transcriptase enzymes / AMV reverse transcriptases

Candidate for the stated function. Confirm substrate, formulation, reaction conditions and application before substitution.

Reverse transcriptase enzymes / AMV reverse transcriptases

AMV-derived reverse transcriptase with intrinsic RNase H activity. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.

Reverse transcriptase enzymes / AMV reverse transcriptases

Native AMV reverse transcriptase retaining RNase H activity. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.

RT master mixes / RT mixes with unspecified priming

See datasheet. 100-test 5× master mix. Distinguish D7182M from the separate-component D7180 kit.

RT master mixes / RT mixes with gDNA removal

See datasheet. 100-test package with a gDNA removal workflow.

RT master mixes / RT mixes with unspecified priming

Pre-primed. gDNA removal and RT are combined in one step; a separate no-RT control mix is included.

First-strand cDNA synthesis kits

First-strand cDNA synthesis reagent kit for reverse transcription of RNA. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.

RT master mixes / RT mixes with gDNA removal

Reverse-transcription premix includes genomic DNA digestion reagents. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.

RT master mixes / RT mixes with gDNA removal

Reverse-transcription premix includes genomic DNA digestion reagents. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.

RT master mixes / Mixed-primer RT mixes

Random primers. Includes random hexamers. Choose LS65 when oligo(dT) is also required.

RT master mixes / Mixed-primer RT mixes

Mixed primers. Contains both random hexamers and oligo(dT). The manufacturer's standard synthesis hold is 42°C.