ABclonal
ABScript III RT Master Mix for qPCR with gDNA Remover
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Mixed primers. Compare the gDNA removal workflow with the standard RK20428 mix.
1st Strand cDNA Synthesis SuperMix (gDNA digester plus, for qPCR) from Arcegen is listed in RT master mixes: RT mixes with gDNA removal.
Pack sizes & catalog numbers
See manufacturer · N132061E
PRODUCT OVERVIEW
Product overview
Reverse-transcription premix includes genomic DNA digestion reagents. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Reverse transcription workflows convert RNA into complementary DNA for downstream amplification or analysis.
For this workflow, compare RNA input, primer composition, genomic DNA removal and downstream assay compatibility. Use the specifications and official documentation below to assess this product against your protocol.
The indexed manufacturer catalog number is N132061E.
- Manufacturer
- Arcegen
- Primary application group
- Reverse transcription
- Reagent category
- RT master mixes / RT mixes with gDNA removal
- SciBranch number
- RM-08.003.004.004-0020-2
- Manufacturer catalog numbers
- N132061E
- Evidence
- Official manufacturer documentation; candidate compatibility requires protocol review
- Other specifications
- Consult manufacturer documentation
Compare with context
Your selection checklist
- 01Primer composition and RNA input
- 02Genomic DNA removal and no-RT controls
- 03Reaction volume and incubation program
- 04Downstream PCR or qPCR compatibility
EXPLORE YOUR OPTIONS
Compare alternatives to 1st Strand cDNA Synthesis SuperMix (gDNA digester plus, for qPCR)
22 cross-manufacturer candidates and 2 same-manufacturer related products.
These products share a candidate substitution group. Interchangeability has not yet been validated against all experimental requirements. Check the listed selection criteria before switching.
Same candidate substitution group
24 productsABclonal
ABScript Neo RT Master Mix for qPCR with gDNA Remover
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Mixed primers. 4× RT mix. A separate remover is added to the same reaction tube.
Applied Biological Materials
All-In-One 5X RT MasterMix with gDNA Removal
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Mixed primers. Premixed temperature-sensitive DNase; the manufacturer specifies a distinct digestion and RT program.
Beyotime
BeyoRT III First Strand cDNA Synthesis Master Mix with gDNA EZeraser
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. 100-test package with a gDNA removal workflow.
Arcegen
Fast 1st Strand cDNA Synthesis SuperMix (gDNA digester plus, for qPCR)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Reverse-transcription premix includes genomic DNA digestion reagents. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Arcegen
Fast RT-gDNA Digestion OneShot SuperMix (for qPCR)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Reverse-transcription premix includes genomic DNA digestion reagents. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Vazyme
HiExact RT SuperMix for qPCR (+gDNA Wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. HiExact-series reverse transcription mix with gDNA removal.
Yeasen
Hifair II 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. Second-generation first-strand supermix package with a genomic-DNA digestion step.
Yeasen
Hifair III 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Mixed primers. 4× Hifair III RT formulation with random primers and oligo(dT)18.
Yeasen
Hifair™ AdvanceFast 1st Strand cDNA Synthesis SuperMix for qPCR (gDNA digester plus)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
First-strand cDNA synthesis premix includes genomic DNA digestion. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Vazyme
HiScript II Q RT SuperMix for qPCR (+gDNA wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Mixed primers. Select the R223 configuration when a gDNA wiper is required.
Vazyme
HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Add primers. Select a random, oligo(dT), or gene-specific primer for the RT step; the kit also includes gDNA removal.
Vazyme
HiScript III RT SuperMix for qPCR (+gDNA wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Pre-primed. A separate 4× gDNA wiper precedes addition of the 5× RT supermix.
Vazyme
HiScript IV RT SuperMix for qPCR (+gDNA wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. Fourth-generation HiScript RT supermix with a gDNA wiper.
Vazyme
HiScript Q RT SuperMix for qPCR (+gDNA wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. Original HiScript Q series with a gDNA wiper workflow.
Vazyme
HisyGo All-in-One RT Red SuperMix for qPCR (+gDNA Wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. All-in-one configuration of the HisyGo RT Red range.
Vazyme
HisyGo RT Red SuperMix for qPCR (+gDNA Wiper)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. Red-format RT mix listed in Vazyme’s qPCR-directed cDNA synthesis catalog.
Bio-Rad
iScript gDNA Clear cDNA Synthesis Kit
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Mixed primers. Contains the iScript RT supermix plus DNase reagents for pretreatment. An alternative kit configuration.
Thermo Scientific
RevertAid Master Mix with DNase I
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Catalog category candidate. Experimental interchangeability has not been validated.
TOYOBO
ReverTra Ace qPCR RT Master Mix with gDNA Remover
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Mixed primers. Sequential DNA removal and RT. TOYOBO specifies its own RT Master Mix II for this workflow.
MedChemExpress
RT Master Mix for qPCR (gDNA digester plus)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Mixed primers. A 2× RT mix follows a separate digestion step. The stock concentration changes the volume added per reaction.
MedChemExpress
RT Master Mix for qPCR II (gDNA digester plus)
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. Second-generation RT formulation supplied for workflows requiring removal of residual genomic DNA.
GDSBio
RT-PCR Mix for qPCR
Read or write a reviewRT master mixes / RT mixes with gDNA removal
Reverse-transcription premix includes genomic DNA digestion reagents. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Invitrogen
SuperScript V VILO Master Mix, Primer-Free, with gDNA Removal
Read or write a reviewPrimer-free RT mixes
Catalog category candidate. Experimental interchangeability has not been validated.
RELATED, NOT INTERCHANGEABLE
These products differ in format, range, formulation or documented enzyme identity. They are shown for further investigation, not as substitutes.
Related options requiring reassessment
29 productsApplied Biological Materials
5X All-In-One RT MasterMix
Read or write a reviewRT master mixes / RT mixes with unspecified priming
Pre-primed. Standard first-strand master mix. Select G592 when the integrated DNase formulation is required.
ABclonal
ABScript III RT Master Mix for qPCR
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. 5× mix for cDNA used in dye- or probe-based qPCR. The manufacturer directs long-amplicon users to another kit.
Bioneer
AccuPower RocketScript Cycle RT PreMix (dT20)
Read or write a reviewRT master mixes / Oligo(dT)-primed RT mixes
Catalog category candidate. Experimental interchangeability has not been validated.
Bioneer
AccuPower RocketScript RT PreMix
Read or write a reviewRT master mixes / Primer-free RT mixes
Catalog category candidate. Experimental interchangeability has not been validated.
Bioneer
AccuPower RocketScript RT PreMix, RNase H Minus
Read or write a reviewRT master mixes / Primer-free RT mixes
Add primers. Use the RNase H-minus format with a separately selected RT primer.
Bioneer
AccuPower RT PreMix
Read or write a reviewRT master mixes / Primer-free RT mixes
Catalog category candidate. Experimental interchangeability has not been validated.
Beyotime
BeyoRT III First Strand cDNA Synthesis Master Mix (5X)
Read or write a reviewRT master mixes / RT mixes with unspecified priming
See datasheet. 100-test 5× master mix. Distinguish D7182M from the separate-component D7180 kit.
TransGen Biotech
TransGen Biotech
EasyScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR
Read or write a reviewRT master mixes / RT mixes with unspecified priming
Pre-primed. gDNA removal and RT are combined in one step; a separate no-RT control mix is included.
NIPPON Genetics EUROPE
FastGene Scriptase II cDNA 5× ReadyMix
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Random primers. Includes random hexamers. Choose LS65 when oligo(dT) is also required.
NIPPON Genetics EUROPE
FastGene Scriptase II cDNA 5× ReadyMix OdT
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. Contains both random hexamers and oligo(dT). The manufacturer's standard synthesis hold is 42°C.
Solis BioDyne
FIREScript RT cDNA Synthesis Mix (without primers)
Read or write a reviewRT master mixes / Primer-free RT mixes
Primer-free RT reaction premix with a separate FIREScript/RNase-inhibitor enzyme mix; selected SKU is the unprimed formulation. Review the manufacturer protocol before substituting.
Vazyme
HiScript II Q RT SuperMix for qPCR
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. Standard HiScript II Q formulation for preparing qPCR templates.
Vazyme
HiScript IV All-in-One Ultra RT SuperMix for qPCR
Read or write a reviewRT master mixes / RT mixes with gDNA removal
See datasheet. Single-reaction gDNA removal and reverse transcription.
Bio-Rad
iScript Reverse Transcription Supermix for RT-qPCR
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. 100 reactions. A matched no-RT control is supplied for control reactions.
New England Biolabs
LunaScript RT SuperMix
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
New England Biolabs
LunaScript RT SuperMix Kit
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
Takara Bio
PrimeScript RT Master Mix (Perfect Real Time)
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. 5× mix containing random hexamers and oligo(dT). The listed 200 reactions use a 10 µL format.
Quantabio
qScript cDNA SuperMix
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. 5× mix for short targets. The vendor lists 10 pg-1 µg total RNA.
Quantabio
qScript XLT cDNA SuperMix
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. Uses an engineered reverse transcriptase with reduced RNase H activity. Check its own incubation program.
Thermo Scientific
RevertAid Master Mix
Read or write a reviewRT master mixes / RT mixes with unspecified priming
Catalog category candidate. Experimental interchangeability has not been validated.
TOYOBO
ReverTra Ace qPCR RT Master Mix
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. 5× mix supplied with no-RT control. Optimized for short cDNA targets in real-time PCR.
MedChemExpress
RT Master Mix for qPCR
Read or write a reviewRT master mixes / RT mixes with unspecified priming
See datasheet. Original first-strand formulation for downstream PCR or qPCR.
MedChemExpress
RT Master Mix for qPCR II
Read or write a reviewRT master mixes / Mixed-primer RT mixes
Mixed primers. 4× Super RT Mix supplied with water. Compare reagent volume when changing from a 5× mix.
Solis BioDyne
SOLIScript RT cDNA Synthesis Mix (without primers)
Read or write a reviewRT master mixes / Primer-free RT mixes
Primer-free RT reaction premix with a separate SOLIScript/RNase-inhibitor enzyme mix; supply application-specific primers. Review the manufacturer protocol before substituting.
Showing 24 of 29
Explore manufacturer resources
Broader catalog resources for this branch. These links are discovery starting points, not verified replacements for individual products.
FROM THE LAB
Researcher ratings and reviews
New
☆☆☆☆☆0 researcher reviews
Be the first to share your experience.
Ratings reflect user-reported experience, not independent performance testing or verified purchases. Reviews are never seeded or generated.
Have you used this reagent?
Create a researcher profile to rate products and share application-specific feedback.
Join the research communityTRACE THE EVIDENCE
Product sources and documentation
Reverse-transcription premix includes genomic DNA digestion reagents. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Official manufacturer product pageArcegen · Product details and documentation
Reverse-transcription premix includes genomic DNA digestion reagents.
Arcegen | 1st Strand cDNA Synthesis SuperMix (gDNA digester plus, for qPCR)Official manufacturer documentation; candidate compatibility requires protocol review · See original documentation for publication date
Primary classification is an editorial interpretation of the product name and cited source. Technical equivalence is not established by category membership. Read our methodology.