Promega
GoTaq 2-Step RT-qPCR System
Read or write a reviewTwo-step RT-PCR kits
Two-step reverse transcription and quantitative PCR system. Kit workflow differs from standalone RT mixes and endpoint RT-PCR kits.
CANDIDATE SUBSTITUTION GROUP
Two-step RT-PCR kits
RM-C-08.003.006Compare Two-step RT-PCR kits within Reverse transcription. This category groups catalog records by experimental role and format.
5 products4 manufacturersFinal category
About Two-step RT-PCR kits
Reverse transcription workflows convert RNA into complementary DNA for downstream amplification or analysis.
When selecting products, compare RNA input, primer composition, genomic DNA removal and downstream assay compatibility.
Products in this final group are candidates for comparison. Check the individual specifications and manufacturer protocols before substituting one product for another.
Products in this category
5 productsEURx
EURx
NG dART RT-PCR kit
Read or write a reviewTwo-step RT-PCR kits
Two-step kit combining first-strand cDNA synthesis and a separate endpoint PCR stage. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.
New England Biolabs
OneTaq RT-PCR Kit
Read or write a reviewTwo-step RT-PCR kits
Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.
EURx
EURx
smART RT-PCR kit
Read or write a reviewTwo-step RT-PCR kits
Two-step kit combining first-strand cDNA synthesis and a separate endpoint PCR stage. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.
RNAConnect
uMRT Two-Step RT-PCR Kit
Read or write a reviewTwo-step RT-PCR kits
Two-step kit provides reverse transcription followed by a separate endpoint PCR amplification workflow. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.
Compare with context
Your selection checklist
- 01Primer composition and RNA input
- 02Genomic DNA removal and no-RT controls
- 03Reaction volume and incubation program
- 04Downstream PCR or qPCR compatibility
ALTERNATIVE DOES NOT MEAN IDENTICAL
What to check before choosing a product
Final groups are designed around experimental substitution requirements. Group membership is provisional until the necessary technical details have been verified for the intended experiment.
Start with your application, then verify the technical requirements against each manufacturer’s documentation.
Latest manufacturer research
Reviewed 2026-11-06. 3 additional products indexed in this pass.
Verified additional manufacturer-listed candidates for this experimental workflow. Product notes state formulation, substrate or platform constraints; substitution requires protocol review. Additional research pass: Verified manufacturer descriptions against the narrow reaction and formulation criteria; existing catalog products were excluded.
Download the latest category evidenceCategory research.
Search did not establish a non-NEB manufacturer product with the same specified function. NEB resale listings excluded.
Download research evidence and coverage gapsExplore manufacturer resources
Broader catalog resources for this branch. These links are discovery starting points, not verified replacements for individual products.