Reverse transcription

RT master mixes / RT mixes with unspecified priming

See datasheet. Original first-strand formulation for downstream PCR or qPCR.

RT master mixes / RT mixes with gDNA removal

Mixed primers. A 2× RT mix follows a separate digestion step. The stock concentration changes the volume added per reaction.

RT master mixes / Mixed-primer RT mixes

Mixed primers. 4× Super RT Mix supplied with water. Compare reagent volume when changing from a 5× mix.

RT master mixes / RT mixes with gDNA removal

See datasheet. Second-generation RT formulation supplied for workflows requiring removal of residual genomic DNA.

First-strand cDNA synthesis kits

First-strand cDNA synthesis reagent kit for reverse transcription of RNA. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.

RT master mixes / RT mixes with gDNA removal

Reverse-transcription premix includes genomic DNA digestion reagents. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.

First-strand cDNA synthesis kits

First-strand cDNA synthesis kit for RNA reverse transcription. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.

Reverse transcriptase enzymes / Engineered reverse transcriptases

Engineered M-MLV reverse transcriptase lacks RNase H activity and supports elevated-temperature cDNA synthesis. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.

Reverse transcriptase enzymes / Engineered reverse transcriptases

Engineered reverse transcriptase with reduced RNase H activity and increased thermal stability. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.

Two-step RT-PCR kits

Two-step kit combining first-strand cDNA synthesis and a separate endpoint PCR stage. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.

Template-switching RT

Engineered MMLV reverse transcriptase with terminal-transferase activity and a documented template-switching protocol for full-length cDNA. Candidate for this workflow; confirm formulation, substrate and protocol compatibility before substitution.

Reverse transcriptase enzymes / Engineered reverse transcriptases

SOLIScript reverse transcriptase with buffer; reduced RNase H activity and operation up to 60 C. Review the manufacturer protocol before substituting.

First-strand cDNA synthesis kits

First-strand synthesis kit using SOLIScript chimeric RT with reduced RNase H activity. Review the manufacturer protocol before substituting.

RT master mixes / Primer-free RT mixes

Primer-free RT reaction premix with a separate SOLIScript/RNase-inhibitor enzyme mix; supply application-specific primers. Review the manufacturer protocol before substituting.

Reverse transcriptase enzymes / Engineered reverse transcriptases

Candidate for the stated function. Confirm substrate, formulation, reaction conditions and application before substitution.

Template-switching RT

Candidate for the stated function. Confirm substrate, formulation, reaction conditions and application before substitution.

RT master mixes / Mixed-primer RT mixes

Mixed primers. 500-reaction pack with no-RT control. The ezDNase bundle, 11766500, adds a DNA-removal step.

RT master mixes / Mixed-primer RT mixes

Catalog category candidate. Experimental interchangeability has not been validated.

Primer-free RT mixes

Catalog category candidate. Experimental interchangeability has not been validated.

RT master mixes / Mixed-primer RT mixes

Random primers. The original VILO uses random primers. IV VILO and V VILO include oligo(dT) as well.

First-strand cDNA synthesis kits

Candidate for the stated function. Confirm substrate, formulation, reaction conditions and application before substitution.

Primer-free RT mixes

Reverse transcription reaction mix is supplied without embedded primers; separate oligo(dT) and random primers allow user-selected priming. Candidate for the listed workflow; compare buffer, sample input and protocol requirements before substitution.

Template-switching RT

Classification is based on the supplied product name. Confirm formulation, application and compatibility in the manufacturer documentation.

RT master mixes / Mixed-primer RT mixes

Mixed primers. Anchored oligo(dT)18 and random 9-mers. Supplied with no-RT control; intended for downstream qPCR.